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MTT colorimetric assay with human NK cell activity

Antonelli. eta1. DistributionofWaturalkillercellActivityinPeripheralBlood, CordBlood. Thymus, LymphNodesandSpleenandtheEffectofinvifroTreatmeatwithInterferonPreparatio-,[link widoczny dla zalogowanych],. Clinlatm & Immu ~ opatho1.1981, 19l161. ● feather fan ● ● ● ● EstablishmentofMTTCoIorimetricAssayforH. umanNKCeIIActivityWangXinjiangLuHua. GuShuyan (DepartmentofImmunology.ChineseAcademyofPreventineMedicine, Instituteo/Virology.Beijing100052) · 73 · ThispaperdescribestheestablishmentofMTTcolorimetricassayfordetectinghumanNKcellactivity. TheinfluencesOilNKactivityweresystematicallyinvestigatedinrespectofdifferentratiosofeffecttotargetcells. kil1ingtime,[link widoczny dla zalogowanych], MTTreactiontimeandtargetcel1cultivationtime. Thedataindicatedthatthismethodhadadvantagesofmanipulating, simplicity, highsensitivityandreproducibility. 22healthyadultsand30healthychildrenwereexaminedwiththismethodfortheirNKcellactivities. Theresu1tswere42.5 ± 15.2% fortheadults, and40.6 ± 14.2forthechildFen. Keywords: MTTcolorimetricassayNKcellactivity Changchun to NHDV Infection of Liu Changqing An Aiping Wang Cheng Xun Shang Lihong Zhao Rui (Children's Hospital in Changchun City,[link widoczny dla zalogowanych], Jilin Province. Changchun 130051) to N275,[link widoczny dla zalogowanych], Changchun, Jilin our HBsAgI ~ of adult patients with asymptomatic carriers and different type of serum hepatitis B patients had hepatitis D virus (HDV) antibody (anti-A HD) detection. The results are as follows: 275NHBsAgI ~ I of serum, 74 cases of adult asymptomatic HBsAg carriers. Small JLHBsAg ~ of 30 patients with adult chronic persistent and chronic active hepatitis B l7l cases. Application of ELISA detection of anti-off l b ~ HD. Test kits from China Academy of Preventive Medicine,[link widoczny dla zalogowanych], Institute of Virology. 275 cases of hepatitis B anti-HBsAg positive sera were detected in a HDI ~ I of 15 cases. 74 cases of adult asymptomatic carriers of HBsAg detection in serum anti-A HD; 30 cases of small JLHBsAgI ~ I detected in the serum of patients with anti-_HD3 cases, accounting for 10%. 17l adult patients with chronic persistent and active hepatitis B anti-serum detected a HDZ2 cases, accounting for 7. The results confirmed the Changchun area in adults and children have hepatitis D virus infection. It is noteworthy that, / bJhHBsAg few positive hepatitis patients, the positive rate of anti _HD in adult chronic persistent and active hepatitis B ~-HDm of the detection rate. The results suggest that, on the HBsAgl ~ l of hepatitis B patients. HD should be a routine anti-testing, for early detection, prompt treatment and prevent expansion of infection and epidemic. Received ll991.02_25. I} return l1991-05-18
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