 Wysłany: Śro 10:12, 06 Kwi 2011 Temat postu: Paliurus structure and content of flavonoid compou |
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| Paliurus structure and content of flavonoid compounds
esofDiozpy-rosKakiLeaves. ChemPharmBull, 1979,27 (11): 2865. THESTRUCTUREANDCONTENTSOFFLAVONOIDSINTHELEAVESOFCYCLOCARYAPALIURUS (BATAL.) ILlINSKXIEMing. yong, YUYin. 1i, WANGYuan. xing, YIXing, WANGXiao-ru (1.TheKeyLaboratoryofFoodScienceofMOE, NanchangUniversity, Nanchang330047, China; 2.TheKeyLaboratoryofAnalyticalScienceofMOE, XiamenUniversity, Xiamen.361005, China) Abstract: ToinvestigatethestructureandcontentsofflavonoidsintheleavesofCyclocaryapaliurus (Bata1.) I ~ insk. Thechemicalcomponentsoftheleaveswereisolatedbysolventextractionandcolumnchromatography. Thechemicalstructureswereidentifiedonthebasisofphysic-chemicalconstantandspectraldata. Thecon-tentsofkaempferol, quercetinandisoquercetinintheleavesweredeterminedbygradientreversed-phasehigh-performanceliquidchromatographyonAlltimaC18column (5pm, 150 × 4.6mm) withUVdetector, atthewavelengthof360nm. Amixtureofwaterandaceticacid (H2O: HOAc98: 2) andacetronitrilewereusedforeluentAandeluentB, respectively. Alineargradientrangedfrom8% to50% foreluentBover30min,adidas scarpe, withaflowrateof0.6mL · min one. Theaveragerecoveryandrelativestandarddeviationofthismethodwere98.6% and1.57%, respectively. Threeflavonoidscompounds: kaempferol (I), quercetin (Ⅱ), andisoquercitrin (HI) wereisolatedforthefirsttimefromtheleavesofCyclocaryapaliurus (Bata1.) Iljinsk. . Theircontentsinthemethanolandwaterextractivesolutionsare: kaempferol (I) 0.0387% and0.0337%, quercetin (Ⅱ) 0.0615% and0.0603% isoquercitrin (HI) 0.543% and0.464%, respectivelyKeywords: Cyclocaryapaliurus (Bata1.) Iljinsk; kaempferol ; Quercetin; isoquercitrin; HPLC |
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