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Wysłany: Czw 6:12, 24 Lut 2011 Temat postu: clarks shoes outlet Pill capsules bone ribs _2571 |
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Bone quality standard bar pill capsules
Solution of 10jxl, respectively, points on the same silica gel G TLC plate, a toluene ethanol, acetone, one of a strong ammonia solution (4:5:0.6:0.4) as the agent, removed, dried, sprayed with dilute bismuth potassium iodide test solution. Test products for chromatography, chromatography with the appropriate position reference substance, the same color of the spots should be less than the reference substance and light spots. 4 4.1 Determination of pieces of column chromatography Services: KromasilCl8 (250mm × 4.6mm, 5tJ.m); mobile phase: methanol water (25:75); detection wavelength: 230nm; flow rate: 1.0ml/min; column temperature: 30%; injection volume: 10txl. 4.2 Relation of precision linear drawing of paeoniflorin concentration 0.0996mg/ml reference solution 1,2,3,4,5 ml, were placed in 10ml volumetric flask, diluted with methanol to the mark, shake, precision learn 10txl injection, measured peak area values to concentration as abscissa and peak area value of the vertical axis, the standard curve, the regression equation of paeoniflorin: A: 1071377.07C +3305.136, r = O . 9996. The results showed that paeoniflorin in the range of 0.0996-0.498 ~ zg good linear relationship. 4,3 stability test were drawn with the precision of a test solution,[link widoczny dla zalogowanych], at 0,3,6,9,12 h sampling,[link widoczny dla zalogowanych], determination of paeoniflorin peak area integral values, the results show that, 12h value within the peak area without large changes, the RSD is 076%. 44 lessons with a precision of precision for the test solution, repeat 5 times injection, determination of paeoniflorin peak area value, the RSD was 0,58%. 4.5 repeatability with the number of samples taken (20,051,021) 5 copies 5 copies of the parallel extraction to determine the average concentration of paeoniflorin was 0.29mg / tablets, RSD = 126%. 4.6 Recovery test of sample has been measured accurately weighed sample content of the same batch number (20051021) 5 copies,[link widoczny dla zalogowanych], each 08g, respectively, by adding the concentration of precision control solution of paeoniflorin 0.0996mg/ml 8ml,[link widoczny dla zalogowanych], then precision by adding methanol 42ml, by determination of the sample under measurement method according to the law, determination of content, and calculate the recovery rate, the results showed that paeoniflorin average recovery was 99.82%, RSD 1.64%. 4.7 lO of determination to take this product the contents of tablets, mix, take about 1.5g, accurately weighed, stoppered flask set, the precision by adding methanol 50ml, said that given the weight, soak overnight, then ultrasonic treatment 30min , let cool, said that given the weight, add methanol to complement the weight loss, shaking, filtration, the filtrate obtained by micro-larvae L membrane (0.45jxm) filtration, were determined by high performance liquid chromatography, the results that lot number 20051011,20051022,20051023 of paeoniflorin were 0.29mg / tablets, 0.30mg / tablets, 0.27rag / tablets. 5 Discussion 5.1 The composition of strains of l4 compound Chinese herbal medicines, composition more complex trial, the exploration, the establishment of Corydalis, white peony root, Panax TLC identification method, thin spots were clear and well separated, and the negative non-interference , pill capsules can be used as quality control of bone tendon qualitative methods. 5.2 The product contains toxic brucine, in order to ensure the safe use of this product, so its toxic components by TLC method for limited inspection of strychnine, Ning limit is 2.20% conversion limit here, the test solution of strychnine in the ceiling should be 1.98mg/ml, so the use of strychnine 2.0mml control solution as control, the test Strychnine chromatographic spots and should be less than the reference substance in the spot light, and thus the product of the effective control of drug safety. 5.3 TGP minister in the Department of Medicine in the product, we use the HPLC method for simultaneous determination of paeoniflorin, paeoniflorin peak and the other components of the sample up to the baseline peak separation, and separation other than the adjacent peaks 2; calculated by paeoniflorin peak, the number of theoretical plates than in 3000, while missing from the Shao-negative solution of paeoniflorin chromatography with reference substance in the corresponding position of peak no peak, indicating no negative interference f,[link widoczny dla zalogowanych], pill capsules can be used as bone ribs quantitative methods.
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